CRISPR/Cas9-mediated cleavage efficiency

Gel image of a cleavage assay using the GeneArt® Genomic Cleavage Detection Assay (Cat. No. A24372) for the HPRT locus. (A) Results using the GeneArt® CRISPR Nuclease OFP Vector expressing HPRT-specific CRISPR RNA. (B) Results obtained using the GeneArt® CRISPR Nuclease CD4 Vector expressing HPRT-specific CRISPR RNA. Following transfection into HeLa cells, triplicate cleavage assays were performed and the percentage of indels were calculated.

Gel image of a cleavage assay using the GeneArt® Genomic Cleavage Detection Assay (Cat. No. A24372) for the HPRT locus. (A) Results using the GeneArt® CRISPR Nuclease OFP Vector expressing HPRT-specific CRISPR RNA. (B) Results obtained using the GeneArt® CRISPR Nuclease CD4 Vector expressing HPRT-specific CRISPR RNA. Following transfection into HeLa cells, triplicate cleavage assays were performed and the percentage of indels were calculated.

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