Immunocytochemistry using Alpha-Tubulin Monoclonal Antibody, Mouse

Anti–bovine α-tubulin, biotin-XX conjugate; Alexa Fluor 647 goat anti–mouse IgG (H+L) conjugate; SYTOX Orange nucleic acid stain. A zebrafish cryosection incubated with the biotin-XX conjugate of mouse monoclonal anti–α-tubulin antibody (Cat. no. A21371). The signal was amplified with TSA Kit #22, which includes HRP–streptavidin and Alexa Fluor 488 tyramide (Cat. no. T20932). The sample was then incubated with the mouse monoclonal FRet 6 antibody and was visualized with Alexa Fluor 647 goat anti–mouse IgG (Cat. no. A21235), which is pseudocolored magenta. Finally, the nuclei were counterstained with SYTOX Orange nucleic acid stain (Cat. no. S11368).

Anti–bovine α-tubulin, biotin-XX conjugate; Alexa Fluor 647 goat anti–mouse IgG (H+L) conjugate; SYTOX Orange nucleic acid stain. A zebrafish cryosection incubated with the biotin-XX conjugate of mouse monoclonal anti–α-tubulin antibody (Cat. no. A21371). The signal was amplified with TSA Kit #22, which includes HRP–streptavidin and Alexa Fluor 488 tyramide (Cat. no. T20932). The sample was then incubated with the mouse monoclonal FRet 6 antibody and was visualized with Alexa Fluor 647 goat anti–mouse IgG (Cat. no. A21235), which is pseudocolored magenta. Finally, the nuclei were counterstained with SYTOX Orange nucleic acid stain (Cat. no. S11368).

Related Products

Sku Name Size Price Qty
A21235 Alexa Fluor® 647 Goat Anti-Mouse IgG (H+L) 0.5 mL USD 149.00
A21371 Alpha-Tubulin Mouse anti-Bovine mAb (clone 236-10501), biotin conjugate 50 µg USD 260.00
S11368 SYTOX® Orange Nucleic Acid Stain - 5 mM Solution in DMSO 250 µL USD 212.00
T20932 TSA™ Kit #22, with HRP—Streptavidin and Alexa Fluor® 488 Tyramide 1 kit USD 466.00

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