Proliferation of muntjac cells detected with Click-iT™ EdU.

Muntjac cells were treated with 10 µM EdU (Cat. No. A10044) for 45 min; cells were then fixed and permeabilized, and EdU that had been incorporated into newly synthesized DNA was detected using the far red–fluorescent Click-iT™ EdU Alexa Fluor® 647 High-Throughput (HCS) Assay (Cat. No. A10208), utilizing the technical tip for converting the HCS assay to conventional fluorescence microscopy. Tubulin was labeled with an anti-α-tubulin antibody (Cat. No. A11126) and visualized with Alexa Fluor® 350 Goat Anti–Mouse IgG (Cat. No. A11045, A21049). The Golgi complex was stained with green-fluorescent Alexa Fluor® 488 conjugate of lectin HPA from Helix pomatia (edible snail) (Cat. No. L11271), and peroxisomes were labeled with an anti-peroxisome antibody and visualized with orange-fluorescent Alexa Fluor® 555 Donkey Anti–Rabbit IgG (Cat. No. A31572).

Muntjac cells were treated with 10 µM EdU (Cat. No. A10044) for 45 min; cells were then fixed and permeabilized, and EdU that had been incorporated into newly synthesized DNA was detected using the far red–fluorescent Click-iT™ EdU Alexa Fluor® 647 High-Throughput (HCS) Assay (Cat. No. A10208), utilizing the technical tip for converting the HCS assay to conventional fluorescence microscopy. Tubulin was labeled with an anti-α-tubulin antibody (Cat. No. A11126) and visualized with Alexa Fluor® 350 Goat Anti–Mouse IgG (Cat. No. A11045, A21049). The Golgi complex was stained with green-fluorescent Alexa Fluor® 488 conjugate of lectin HPA from <i>Helix pomatia</i> (edible snail) (Cat. No. L11271), and peroxisomes were labeled with an anti-peroxisome antibody and visualized with orange-fluorescent Alexa Fluor® 555 Donkey Anti–Rabbit IgG (Cat. No. A31572).

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A10044 EdU (5-ethynyl-2’-deoxyuridine) 50 mg USD 383.00
A11045 Alexa Fluor® 350 Goat Anti-Mouse IgG (H+L) 0.5 mL USD 149.00
A21049 Alexa Fluor® 350 Goat Anti-Mouse IgG (H+L), highly cross-adsorbed 0.5 mL USD 199.00
A31572 Alexa Fluor® 555 Donkey Anti-Rabbit IgG (H+L) Antibody 0.5 mL USD 189.00
E10187 EdU (5-ethynyl-2’-deoxyuridine) 500 mg USD 1,174.00
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L11271 Lectin HPA From Helix pomatia (edible snail), Alexa Fluor® 488 Conjugate 1 mg USD 215.00

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