Immunohistochemistry using rabbit anti-nitrotyrosine polyclonal antibody.
Nitrated tyrosine residues in bovine pulmonary artery endothelial cells detected with rabbit anti-nitrotyrosine antibody. Fixed and permeabilized bovine pulmonary artery endothelial cells were treated with either degraded peroxynitrite (top panel) or 100 µM peroxynitrite (bottom panel) for five minutes at room temperature to induce protein nitration. Nitrated tyrosine residues were detected with our rabbit anti-nitrotyrosine polyclonal antibody (Cat. No. A21285) and visualized with the green-fluorescent Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. No. A11008). Nuclei were counterstained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490).