NIH 3T3 cells showing incorporation of BrdU.
Cells were pulsed with BrdU (Cat. No. B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (Cat. No. A21300) and green-fluorescent Alexa Fluor® 488 Goat Anti–Mouse IgG1 isotype–specific secondary antibody (Cat. No. A21121); the BrdU staining pattern is co-localized with the nuclear staining of blue-fluorescent DAPI (Cat. No. D1306, D21490). Mitochondria were labeled with anti-COX, Complex IV, subunit I, and red-fluorescent Alexa Fluor® 594 Goat Anti–Mouse IgG2a isotype-specific antibody (Cat. No. A21135). Coverslips were mounted with Prolong® antifade mounting medium (Cat. No. P7481).
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›
Endothelial cells. Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
Phagocytosis in MMM Cells using pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) Go ›
Muntjac skin fibroblast labeled with rhodamine phalloidin. Go ›
Performance of the Qubit® dsDNA HS Assay Go ›
Low protein-to-protein variation in the Qubit® protein assay Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Nuclear, cytoskeletal, and mitochondrial staining of Muntjac fibroblasts. Go ›