Macrophages cultured on a polymer surface that have fused to form a foreign-body giant cell following treatment with interleukin-4.

Macrophages cultured on a polymer surface that have fused to form a foreign-body giant cell following treatment with interleukin-4. Cells were fixed with 3.7% formaldehyde, treated with RNase A and stained with rhodamine phalloidin (Cat. no. R415) to visualize F-actin, and with YO-PRO®-1 iodide (Cat. no. Y3603) to visualize cell nuclei. Cells were imaged with a Bio-Rad MRC600 confocal laser-scanning microscope. The image is a composite of optical sections taken through the Z-axis of the cell. F-actin (red) is restricted to the periphery of the multinucleated cell and surrounds a cluster of nuclei (green). Image contributed by Kristin DeFife and James M. Anderson, Institute of Pathology, Case Western Reserve University.

Macrophages cultured on a polymer surface that have fused to form a foreign-body giant cell following treatment with interleukin-4. Cells were fixed with 3.7% formaldehyde, treated with RNase A and stained with rhodamine phalloidin (Cat. no. R415) to visualize F-actin, and with YO-PRO®-1 iodide (Cat. no. Y3603) to visualize cell nuclei. Cells were imaged with a Bio-Rad MRC600 confocal laser-scanning microscope. The image is a composite of optical sections taken through the Z-axis of the cell. F-actin (red) is restricted to the periphery of the multinucleated cell and surrounds a cluster of nuclei (green). Image contributed by Kristin DeFife and James M. Anderson, Institute of Pathology, Case Western Reserve University.

Related Products

Sku Name Size Price Qty
R415 Rhodamine Phalloidin 300 units USD 359.00
Y3603 YO-PRO®-1 Iodide (491/509) 1 mL USD 268.00

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