HeLa cell nuclei. Texas Red-X streptavidin, biotin-XX goat anti–mouse IgG antibody, ELF 97 Cytological Labeling Kit and Hoechst 33258.

HeLa cell nuclei incubated with a mouse monoclonal antibody directed against nuclear-localized antigens (a gift from Benjamin Blencowe and Phil Sharp, Massachusetts Institute of Technology) in conjunction with Texas Red-X streptavidin (S6370) and with a human anti-nuclear antibody in conjunction with biotin-XX goat anti–human IgG antibody. The reagents in the ELF 97 Cytological Labeling Kit (E6603) were then used to detect the biotinylated secondary antibody. Cells were counterstained with Hoechst 33258 (H1398, H3569, H21491). Multiple exposures were taken using filters appropriate for the Texas Red dye, fluorescein and DAPI.

HeLa cell nuclei incubated with a mouse monoclonal antibody directed against nuclear-localized antigens (a gift from Benjamin Blencowe and Phil Sharp, Massachusetts Institute of Technology) in conjunction with Texas Red-X streptavidin (S6370) and with a human anti-nuclear antibody in conjunction with biotin-XX goat anti–human IgG antibody. The reagents in the ELF 97 Cytological Labeling Kit (E6603) were then used to detect the biotinylated secondary antibody. Cells were counterstained with Hoechst 33258 (H1398, H3569, H21491). Multiple exposures were taken using filters appropriate for the Texas Red dye, fluorescein and DAPI.

Related Products

Sku Name Size Price Qty
S6370 Streptavidin, Texas Red®-X conjugate 1 mg USD 165.00
H1398 Hoechst 33258, Pentahydrate (bis-Benzimide), 100 mg 100 mg USD 63.25
H21491 Hoechst 33258, Pentahydrate (bis-Benzimide) - FluoroPure™ Grade 100 mg USD 74.00
H3569 Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg⁄mL Solution in Water 10 mL USD 84.00

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