Bovine pulmonary artery endothelial cell (BPAEC). Oregon Green® 488 phalloidin and Texas Red® deoxyribonuclease I.
Simultaneous visualization of F- and G-actin in a bovine pulmonary artery endothelial cell (BPAEC) using F-actin–specific Oregon Green® 488 phalloidin (Cat. no. O7466) and G-actin–specific Texas Red® deoxyribonuclease I (Cat. no. D972). The G-actin appears as diffuse red fluorescence that is more intense in the nuclear region where the cell thickness is greater and stress fibers are less dense. The image was obtained by taking multiple exposures through bandpass optical filter sets appropriate for fluorescein and the Texas Red® dye.