Transformed mouse fibroblasts (CRE BAG 2 cells).

lacZ mRNA in transformed mouse fibroblasts (CRE BAG 2 cells) are hybridized with a singly biotinylated, complementary oligonucleotide. Hybrids were then detected by incubation with a streptavidin–alkaline phosphatase conjugate in combination with the ELF 97 alkaline phosphatase substrate, both of which are provided in our ELF® 97 mRNA In Situ Hybridization Kit (Cat. No. E6604, E6605). Cells were counterstained with DAPI (Cat. No. D1306, D3571, D21490) and photographed using a longpass optical filter appropriate for DAPI.

<i>lacZ</i> mRNA in transformed mouse fibroblasts (CRE BAG 2 cells) are hybridized with a singly biotinylated, complementary oligonucleotide. Hybrids were then detected by incubation with a streptavidin–alkaline phosphatase conjugate in combination with the ELF 97 alkaline phosphatase substrate, both of which are provided in our ELF® 97 mRNA <i>In Situ</i> Hybridization Kit (Cat. No. E6604, E6605). Cells were counterstained with DAPI (Cat. No. D1306, D3571, D21490) and photographed using a longpass optical filter appropriate for DAPI.

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Sku Name Size Price Qty
D1306 DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) 10 mg USD 89.00
D21490 DAPI, FluoroPure™ grade 10 mg USD 133.00
D3571 DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) 10 mg USD 89.00
E6604 ELF® 97 mRNA In Situ Hybridization Kit #1 1 kit USD 356.00

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