Golgi apparatus. BODIPY FL C5-ceramide
Selective staining of the Golgi apparatus using the green-fluorescent BODIPY FL C5-ceramide (D3521) (top panel). At high concentrations, the BODIPY FL fluorophore forms excimers that can be visualized using a red longpass optical filter (bottom panel). The BODIPY FL C5-ceramide accumulation in the trans-Golgi is sufficient for excimer formation J Cell Biol (1991) 113:1267). Images contributed by Richard Pagano, Mayo Foundation.
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›
Fixed and stained HDFn cells. Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›