Golgi apparatus. BODIPY FL C5-ceramide
Selective staining of the Golgi apparatus using the green-fluorescent BODIPY FL C5-ceramide (D3521) (top panel). At high concentrations, the BODIPY FL fluorophore forms excimers that can be visualized using a red longpass optical filter (bottom panel). The BODIPY FL C5-ceramide accumulation in the trans-Golgi is sufficient for excimer formation J Cell Biol (1991) 113:1267). Images contributed by Richard Pagano, Mayo Foundation.
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›
Fixed and stained HDFn cells. Go ›
Enhanced chromogenic substrate detection using Thermo Scientific Pierce Western Blot Signal Enhancer Go ›
Enhanced chemiluminescent substrate detection using Thermo Scientific Pierce Western Blot Signal Enhancer Go ›