Rat ovarian granulosa cells.
Selective uptake of cholesteryl esters (CE) in rat ovarian granulosa cells as monitored with cholesteryl BODIPY® FL C12 (Cat. No. C3927MP). The hormone-stimulated cells internalized and stored CEs derived from reconstituted high-density lipoprotein (HDL)–BODIPY® CE complexes (J Biol Chem 271, 16208 (1996)). A low-light (<100 µW beam power) computerized imaging system minimized any photobleaching of the fluorophore. This pseudocolored image uses yellow-green to illustrate the low-level fluorescence of the cytoplasmic membranes, yellow to illustrate the medium-level fluorescence of the Golgi, and red to illustrate the high-level fluorescence of the lipid droplets. Image contributed by Eve Reaven, VA Medical Center, Palo Alto, California.
Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody Go ›
Fixed and stained HDFn cells. Go ›
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate. Go ›
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI. Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›