Bovine pulmonary artery endothelial cells. Anti–dinitrophenyl-KLH antibody and LIVE/DEAD® Reduced Biohazard Viability/Cytotoxicity Kit.
A mixed population of trinitrophenyl-sensitized and nonsensitized bovine pulmonary artery endothelial cells that have been probed with anti–dinitrophenyl-KLH antibody (Cat. no. A6430), incubated with rabbit complement and then stained using the LIVE/DEAD® Reduced Biohazard Viability/Cytotoxicity Kit #1 (Cat. no. L7013). With this kit, the membrane-permeant SYTO® 10 dye labels the nucleic acids of live cells with green fluorescence, and the membrane-impermeant DEAD Red™ dye labels nucleic acids of membrane-compromised cells with red fluorescence. Subsequent aldehyde-based fixation inactivates pathogens without distorting the original staining pattern. This multiple-exposure image was acquired with bandpass optical filters appropriate for fluorescein and Texas Red® dyes.
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›