The ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye.

The yellow-green fluorescence of the ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye. Adult zebrafish cryosections were first stained with Texas Red®-X wheat germ agglutinin (Cat. no. W21405) followed by the ELF® 97 phosphate substrate in the ELF® 97 Endogenous Phosphatase Detection Kit (Cat. no. E6601), and finally the nuclei were counterstained with the blue-fluorescent Hoechst 33342 nucleic acid stain (Cat. no. H1399, H3570, H21492). The triple-exposure image was acquired using bandpass filters appropriate for the Texas Red® dye, ELF® 97 alcohol precipitate and AMCA.

The yellow-green fluorescence of the ELF® 97 alcohol precipitate, demonstrating localization of endogenous alkaline phosphatase activity in the ciliary body of the zebrafish eye. Adult zebrafish cryosections were first stained with Texas Red®-X wheat germ agglutinin (Cat. no. W21405) followed by the ELF® 97 phosphate substrate in the ELF® 97 Endogenous Phosphatase Detection Kit (Cat. no. E6601), and finally the nuclei were counterstained with the blue-fluorescent Hoechst 33342 nucleic acid stain (Cat. no. H1399, H3570, H21492). The triple-exposure image was acquired using bandpass filters appropriate for the Texas Red® dye, ELF® 97 alcohol precipitate and AMCA.

Related Products

Sku Name Size Price Qty
E6601 ELF® 97 Endogenous Phosphatase Detection Kit 1 kit USD 273.00
H1399 Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg 100 mg USD 63.25
W21405 Wheat Germ Agglutinin, Texas Red®-X Conjugate 1 mg USD 131.00

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