Endothelial cells.

Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with our mouse monoclonal anti–a-tubulin antibody (Cat. No. A11126), detected with the biotin-XX–conjugated F(ab')2 fragment of goat anti–mouse IgG antibody (Cat. No. B11027) and visualized with Alexa Fluor® 488 streptavidin (Cat. No. S11223). The actin filaments were then labeled with orange-fluorescent Alexa Fluor® 568 phalloidin (Cat. No. A12380), and the cell was counterstained with blue-fluorescent Hoechst 33342 (Cat. No. H1399, H3570, H21492) to image the DNA, and red-fluorescent propidium iodide (Cat. No. P1304MP, P3566, P21493) to image the nucleolar RNA. The multiple-exposure image was acquired using bandpass filters appropriate for the Texas Red® dye, fluorescein, and DAPI.

Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with our mouse monoclonal anti–a-tubulin antibody (Cat. No. A11126), detected with the biotin-XX–conjugated F(ab')<sub>2</sub> fragment of goat anti–mouse IgG antibody (Cat. No. B11027) and visualized with Alexa Fluor® 488 streptavidin (Cat. No. S11223). The actin filaments were then labeled with orange-fluorescent Alexa Fluor® 568 phalloidin (Cat. No. A12380), and the cell was counterstained with blue-fluorescent Hoechst 33342 (Cat. No. H1399, H3570, H21492) to image the DNA, and red-fluorescent propidium iodide (Cat. No. P1304MP, P3566, P21493) to image the nucleolar RNA.  The multiple-exposure image was acquired using bandpass filters appropriate for the Texas Red® dye, fluorescein, and DAPI.

Related Products

Sku Name Size Price Qty
A11126 Bovine Alpha-Tubulin Mouse mAb (clone 236-10501) 50 µg USD 200.00
A12380 Alexa Fluor® 568 Phalloidin 300 units USD 373.00
B11027 Biotin-XX F(ab')2 Fragment of Goat Anti-Mouse IgG (H+L) 250 µL USD 119.00
H1399 Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg 100 mg USD 63.25
I37161 Image-iT® FX Kit #12 (with Alexa Fluor® 488 Streptavidin, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
P1304MP Propidium Iodide 100 mg USD 129.00
S11223 Streptavidin, Alexa Fluor® 488 conjugate 1 mg USD 185.00

Related Images