Bovine pulmonary artery endothelial cells.
The subcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells are visualized with several fluorescent dyes. Filamentous actin (F-actin) was identified with Alexa Fluor 633 phalloidin (Cat. No. A22284), which is pseudocolored magenta. Lipophilic regions of the cell, including intracellular membranes, were stained with green-fluorescent DiOC6(3) (Cat. No. D273). Finally, nuclei were counterstained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). The image was acquired using filters appropriate for fluorescein and DAPI and a special filter (courtesy of Omega Optical) for the Alexa Fluor 633 dye, consisting of a narrow band exciter (630DF10), dichroic (640DRLP) and emitter (660DF10).
Developing Drosophila embryo Go ›
Immunohistochemistry using GFAP Monoclonal Antibody, Mouse, Alexa Fluor® 594 Conjugate Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Nuclear, cytoskeletal, and mitochondrial staining of Muntjac fibroblasts. Go ›
Immunofluorescent Staining using Mouse HuC/HuD Neuronal Protein Monoclonal Antibody, Biotin-XX Conjugate. Go ›
Neural somata in mouse cerebellum labeled with NeuroTrace® 530/615 red-fluorescent Nissl stain and DAPI. Go ›