Bovine pulmonary artery endothelial cells.

The subcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells are visualized with several fluorescent dyes. Filamentous actin (F-actin) was identified with Alexa Fluor 633 phalloidin (Cat. No. A22284), which is pseudocolored magenta. Lipophilic regions of the cell, including intracellular membranes, were stained with green-fluorescent DiOC6(3) (Cat. No. D273). Finally, nuclei were counterstained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). The image was acquired using filters appropriate for fluorescein and DAPI and a special filter (courtesy of Omega Optical) for the Alexa Fluor 633 dye, consisting of a narrow band exciter (630DF10), dichroic (640DRLP) and emitter (660DF10).

The subcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells are visualized with several fluorescent dyes. Filamentous actin (F-actin) was identified with Alexa Fluor 633 phalloidin (Cat. No. A22284), which is pseudocolored magenta. Lipophilic regions of the cell, including intracellular membranes, were stained with green-fluorescent DiOC<sub>6</sub>(3) (Cat. No. D273). Finally, nuclei were counterstained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). The image was acquired using filters appropriate for fluorescein and DAPI and a special filter (courtesy of Omega Optical) for the Alexa Fluor 633 dye, consisting of a narrow band exciter (630DF10), dichroic (640DRLP) and emitter (660DF10).

Related Products

Sku Name Size Price Qty
A22284 Alexa Fluor® 633 Phalloidin 300 units USD 373.00
D1306 DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) 10 mg USD 89.00
D21490 DAPI, FluoroPure™ grade 10 mg USD 133.00
D3571 DAPI (4',6-Diamidino-2-Phenylindole, Dilactate) 10 mg USD 89.00
D273 DiOC6(3) (3,3'-Dihexyloxacarbocyanine Iodide) 100 mg USD 167.00

Related Images