Photobleaching of fluorescein phalloidin in bovine pulmonary artery endothelial cells with and without the ProLong® Antifade mounting medium.
Bovine pulmonary artery endothelial cells were labeled with fluorescein phalloidin (Cat. no. F432), which labels filamentous actin, and placed under constant illumination on the microscope with a FITC filter set using a 60× objective. Images were acquired at one-second intervals for 30 seconds. Under these illumination conditions, fluorescein photobleached to about 12% of its initial value in 30 seconds in PBS (left), but stayed at the initial value under the same illumination conditions when mounted using the reagents in the ProLong® Antifade Kit (right, Cat. no. P7481).
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›
Phagocytosis in MMM Cells using pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) Go ›
Muntjac skin fibroblast labeled with rhodamine phalloidin. Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›