NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
NIH 3T3 cells that were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, then plated and allowed to attach and proliferate. The cells were fixed and labeled with our Alexa Fluor® 594 conjugate of the anti-GFP antibody (Cat. no. A21312). Cells expressing GFP show dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI (Cat. no. D1306, D3571, D21490) stains the nuclei with a light-blue fluorescence. In the cells that are not transfected, the DAPI-stained nuclei exhibit a bright blue fluorescence.
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Developing Drosophila embryo Go ›
Immunohistochemistry using GFAP Monoclonal Antibody, Mouse, Alexa Fluor® 594 Conjugate Go ›
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain. Go ›
2D gel stained with the SYPRO Ruby protein gel stain and the Pro-Q Emerald 300 reagent. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Porcine primary skeletal muscle cell transduced with Organelle Lights fluorescent proteins. Go ›
Indirect ELISA analysis of ABfinity™ Troponin I Recombinant Rabbit Monoclonal Antibody Go ›
Sandwich ELISA analysis of ABfinity™ EGF Recombinant Rabbit Monoclonal Antibody Go ›
Western blot analysis of (A) MDA-MB-231, (B) MDA-MB-468, (C) TF-1, (D) K562, (E) Caco-2, and (F) PC-3 cell lysates, and (G) mouse testis homogenates using our Rb anti-Metadherin (C-term) (Cat. No. 40-6400). Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›