NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.

NIH 3T3 cells that were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, then plated and allowed to attach and proliferate. The cells were fixed and labeled with our Alexa Fluor® 594 conjugate of the anti-GFP antibody (Cat. no. A21312). Cells expressing GFP show dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI (Cat. no. D1306, D3571, D21490) stains the nuclei with a light-blue fluorescence. In the cells that are not transfected, the DAPI-stained nuclei exhibit a bright blue fluorescence.

NIH 3T3 cells that were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, then plated and allowed to attach and proliferate. The cells were fixed and labeled with our Alexa Fluor® 594 conjugate of the anti-GFP antibody (Cat. no. A21312).  Cells expressing GFP show dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI (Cat. no. D1306, D3571, D21490) stains the nuclei with a light-blue fluorescence. In the cells that are not transfected, the DAPI-stained nuclei exhibit a bright blue fluorescence.

Related Products

Sku Name Size Price Qty
A11122 GFP Rabbit IgG Polyclonal Antibody Fraction 100 µL USD 310.00
A21312 GFP Rabbit IgG Antibody Fraction, Alexa Fluor® 594 Conjugate 100 µL USD 310.00
D1306 DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) 10 mg USD 89.00
D21490 DAPI, FluoroPure™ grade 10 mg USD 133.00

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