Live bovine pulmonary artery endothelial cells (BPAEC) stained with LysoTracker® Red DND-99, dihydrorhodamine 123 and Hoechst 33258.

Live bovine pulmonary artery endothelial cells (BPAEC) were first stained with LysoTracker® Red DND-99 (Cat. no. L7528). Then, a solution of dihydrorhodamine 123 (Cat. no. D632, D23806) and Hoechst 33258 (Cat. no. H1398, H3569, H21491) was added and allowed to incubate with the cells for an additional 10 minutes before the cells were subsequently washed and visualized. The green-fluorescent oxidation product (rhodamine 123, Cat. no. R302) localized primarily to the mitochondria. The red-fluorescent LysoTracker® Red DND-99 stain accumulated in the lysosomes, and the blue-fluorescent Hoechst 33258 dye stained the nuclei. The image was acquired with filters appropriate for DAPI, fluorescein and the Texas Red® dye. The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/). 3-D reconstruction was performed using Imaris software (Bitplane AG, http://www.bitplane.com/).

Live bovine pulmonary artery endothelial cells (BPAEC) were first stained with LysoTracker® Red DND-99 (Cat. no. L7528). Then, a solution of dihydrorhodamine 123 (Cat. no. D632, D23806) and Hoechst 33258 (Cat. no. H1398, H3569, H21491) was added and allowed to incubate with the cells for an additional 10 minutes before the cells were subsequently washed and visualized.  The green-fluorescent oxidation product (rhodamine 123, Cat. no. R302) localized primarily to the mitochondria. The red-fluorescent LysoTracker® Red DND-99 stain accumulated in the lysosomes, and the blue-fluorescent Hoechst 33258 dye stained the nuclei. The image was acquired with filters appropriate for DAPI, fluorescein and the Texas Red® dye. The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/). 3-D reconstruction was performed using Imaris software (Bitplane AG, http://www.bitplane.com/).

Related Products

Sku Name Size Price Qty
D23806 Dihydrorhodamine 123 1 mL USD 193.00
D632 Dihydrorhodamine 123 10 mg USD 205.00
H1398 Hoechst 33258, Pentahydrate (bis-Benzimide), 100 mg 100 mg USD 63.25
H21491 Hoechst 33258, Pentahydrate (bis-Benzimide) - FluoroPure™ Grade 100 mg USD 74.00
H3569 Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg⁄mL Solution in Water 10 mL USD 84.00
L7528 LysoTracker® Red DND-99 - Special Packaging 20 x 50 µL USD 290.00
R302 Rhodamine 123, 25 mg 25 mg USD 89.00

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