Bovine pulmonary artery endothelial cells (BPAEC) stained with 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, FM 5-95 and Hoechst 33342.

Bovine pulmonary artery endothelial (BPAEC) cells were initially stained with the reactive oxygen species (ROS) indicator, 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C2938). After a 30-minute incubation, the cells were washed and then incubated simultaneously with FM 5-95 (T23360) and Hoechst 33342 (H1399, H3570, H21492) in phosphate-buffered saline (PBS) for an additional five minutes before washing and mounting in PBS. The red-fluorescent FM 5-95 appears to stain both the plasma membrane and early endosomes; the green-fluorescent, oxidized carboxydichlorofluorescein localizes to the cytoplasm; and the blue-fluorescent Hoechst 33342 dye stains the nucleus.

Bovine pulmonary artery endothelial (BPAEC) cells were initially stained with the reactive oxygen species (ROS) indicator, 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C2938). After a 30-minute incubation, the cells were washed and then incubated simultaneously with FM 5-95 (T23360) and Hoechst 33342 (H1399, H3570, H21492) in phosphate-buffered saline (PBS) for an additional five minutes before washing and mounting in PBS. The red-fluorescent FM 5-95 appears to stain both the plasma membrane and early endosomes; the green-fluorescent, oxidized carboxydichlorofluorescein localizes to the cytoplasm; and the blue-fluorescent Hoechst 33342 dye stains the nucleus.

Related Products

Sku Name Size Price Qty
C2938 Di(Acetoxymethyl Ester) (6-Carboxy-2',7'-Dichlorodihydrofluorescein Diacetate) 5 mg USD 257.00
H1399 Hoechst 33342, Trihydrochloride, Trihydrate, 100 mg 100 mg USD 63.25
H21492 Hoechst 33342, Trihydrochloride, Trihydrate - FluoroPure™ Grade 100 mg USD 75.00
H3570 Hoechst 33342, Trihydrochloride, Trihydrate - 10 mg⁄mL Solution in Water 10 mL USD 84.00
T23360 FM® 5-95 (N-(3-Trimethylammoniumpropyl)-4-(6-(4-(Diethylamino)phenyl)hexatrienyl)Pyridinium Dibromide) 1 mg USD 308.00

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