Reversible binding by DSB-X biotin.

Reversible binding by DSB-X biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti–a-tubulin antibody (A11126), detected with either biotin-XX goat anti–mouse IgG antibody (B2763, panel A) or DSB-X biotin goat anti–mouse IgG antibody (D20691, panel B) and visualized with green-fluorescent Alexa Fluor 488 streptavidin (S11223). Nuclei were stained with blue-fluorescent DAPI (D1306, D3571, D21490). After incubating with 10 mM D-biotin (B1595, B20656), the binding between the biotinylated antibody is unaltered (panel C), whereas the streptavidin conjugate has been stripped from the DSB-X biotin–labeled antibody (panel D).

Reversible binding by DSB-X biotin. Microtubules of fixed bovine pulmonary artery endothelial cells were labeled with mouse monoclonal anti–a-tubulin antibody (A11126), detected with either biotin-XX goat anti–mouse IgG antibody (B2763, panel A) or DSB-X biotin goat anti–mouse IgG antibody (D20691, panel B) and visualized with green-fluorescent Alexa Fluor 488 streptavidin (S11223). Nuclei were stained with blue-fluorescent DAPI (D1306, D3571, D21490). After incubating with 10 mM D-biotin (B1595, B20656), the binding between the biotinylated antibody is unaltered (panel C), whereas the streptavidin conjugate has been stripped from the DSB-X biotin–labeled antibody (panel D).

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Sku Name Size Price Qty
A11126 Bovine Alpha-Tubulin Mouse mAb (clone 236-10501) 50 µg USD 200.00
B1595 D-Biotin 1 g USD 74.00
B20656 D-Biotin 10 mL USD 83.00
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D21490 DAPI, FluoroPure™ grade 10 mg USD 133.00
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I37161 Image-iT® FX Kit #12 (with Alexa Fluor® 488 Streptavidin, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
S11223 Streptavidin, Alexa Fluor® 488 conjugate 1 mg USD 185.00

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