Reduced background staining afforded by Image-iT® FX signal enhancer.

Reduced background staining afforded by Image-iT® FX signal enhancer. Mouse brain cryosections were permeabilized and antigen retrieval was carried out. The sections were then treated for 30 minutes with Image-iT® FX signal enhancer (Cat. no. I36933, left) or left untreated (right). Sections were labeled with the neural cell body selective antibody anti–Hu C/D (Cat. no. A21271) and visualized using TSA Kit #2 (Cat. no. T20912) with the HRP conjugate of goat anti–mouse IgG and Alexa Fluor® 488 tyramide. Sections were mounted using the reagents in the ProLong® Antifade Kit (Cat. no. P7481).

Reduced background staining afforded by Image-iT® FX signal enhancer. Mouse brain cryosections were permeabilized and antigen retrieval was carried out. The sections were then treated for 30 minutes with Image-iT® FX signal enhancer (Cat. no. I36933, left) or left untreated (right). Sections were labeled with the neural cell body selective antibody anti–Hu C/D (Cat. no. A21271) and visualized using TSA Kit #2 (Cat. no. T20912) with the HRP conjugate of goat anti–mouse IgG and Alexa Fluor® 488 tyramide. Sections were mounted using the reagents in the ProLong® Antifade Kit (Cat. no. P7481).

Related Products

Sku Name Size Price Qty
A21271 HuC/HuD Neuronal Protein Mouse Monoclonal Antibody (clone 16A11) 100 µg USD 368.00
I36933 Image-iT® FX Signal Enhancer 10 mL USD 98.75
I37150 Image-iT® FX Kit #1 (with Alexa Fluor® 350 Goat Anti-Mouse IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37151 Image-iT® FX Kit #2 (with Alexa Fluor® 488 Goat Anti-Mouse IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37152 Image-iT® FX Kit #3 (with Alexa Fluor® 555 Goat Anti-Mouse IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37153 Image-iT® FX Kit #4 (with Alexa Fluor® 594 Goat Anti-Mouse IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37154 Image-iT® FX Kit #5 (with Alexa Fluor® 647 Goat Anti-Mouse IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37155 Image-iT® FX Kit #6 (with Alexa Fluor® 350 Goat Anti-Rabbit IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37156 Image-iT® FX Kit #7 (with Alexa Fluor® 488 Goat Anti-Rabbit IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37157 Image-iT® FX Kit #8 (with Alexa Fluor® 555 Goat Anti-Rabbit IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37158 Image-iT® FX Kit #9 (with Alexa Fluor® 594 Goat Anti-Rabbit IgG, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37160 Image-iT® FX Kit #11 (with Alexa Fluor® 350 Streptavidin, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37161 Image-iT® FX Kit #12 (with Alexa Fluor® 488 Streptavidin, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37162 Image-iT® FX Kit #13 (with Alexa Fluor® 555 Streptavidin, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37163 Image-iT® FX Kit #14 (with Alexa Fluor® 594 Streptavidin, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 400.00
I37164 Image-iT® FX Kit #15 (with Alexa Fluor® 647 Streptavidin, ProLong® Gold Antifade, and Image-iT® FX Signal Enhancer) 1 kit USD 461.00
P7481 ProLong® Antifade Kit 1 kit USD 241.00
T20912 TSA™ Kit #2, with HRP—Goat Anti-Mouse IgG and Alexa Fluor® 488 Tyramide 1 kit USD 466.00

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