Amplification of the GFP signal localized in mitochondria of a transfected HeLa cell.
HeLa cell transfected with pShooter pCMV/myc/mito/GFP, then fixed and permeabilized. Green-fluorescent protein (GFP) localized in the mitochondria was labeled with anti-GFP mouse IgG2a (Cat. no. A11120) and detected with orange-fluorescent Alexa Fluor® 555 goat anti–mouse IgG (CAt. no. A21422), which colocalized with the dim GFP fluorescence. F-actin was labeled with green-fluorescent Alexa Fluor® 488 phalloidin (Cat. no. A12379), and the nucleus was stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The sample was mounted using ProLong® Gold antifade reagent (Cat. no. P36930). Some GFP fluorescence is retained in the mitochondria after fixation (top), but immunolabeling and detection greatly improve visualization (bottom).
Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›
Endothelial cells. Go ›
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›
Muntjac fibroblast labeled with probes for actin and the nucleus. Go ›
Multicolor fluorescence analysis of muntjac fibroblasts. Go ›
Nuclear, cytoskeletal, and mitochondrial staining of Muntjac fibroblasts. Go ›
Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›
Human tonsil section visualized with fluorescent Qdot® nanocrystal conjugates. Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Porcine primary skeletal muscle cell transduced with Organelle Lights fluorescent proteins. Go ›