Calcium flux in porcine left atrial appendage progenitor cells transfected with Premo™ Cameleon calcium sensor.
Porcine left atrial appendage progenitor cells were transfected with Premo™ Cameleon calcium sensor (P36207, P36208); ATP (20 µM final concentration) was applied to the cells the following day and the cells were imaged using a Zeiss 5 Live high-speed confocal system (Carl Zeiss MicroImaging). Excitation was with a 405 nm diode laser (50 mw) operated at 50% power. Emission was collected simultaneously on two linear CCD detectors using a 490 nm dichroic mirror to split the beam through a 415–480 nm bandpass filter for CFP and a 550 nm longpass filter for YFP. Images were collected at a rate of 10 frames per second (512 x 512 pixels) using a 40x Plan-Neofluar 1.3 NA oil immersion objective lens.
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›
HeLa cells transduced with Organelle Lights™ Mito-GFP. Go ›
Four-color staining of a muntjac cell with probes for cytoskeletal, nuclear and mitochondrial proteins Go ›
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran. Go ›
Phagocytosis in MMM Cells using pHrodo™ Red E. coli BioParticles® Conjugate® (Catalog # P35361) Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Porcine primary skeletal muscle cell transduced with Organelle Lights fluorescent proteins. Go ›
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›